3′-End labeling of nucleic acids by a polymerase ribozyme. Issue 17 (13th June 2018)

• Record Type: Journal Article
• Authors: Samanta, Biswajit; Horning, David P; Joyce, Gerald F
• Is Part Of: Nucleic acids research. Volume 46:Issue 17(2018)
• Page Start: e103
• Page End: e103
• Publication Date: 2018-06-13
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gky513
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: A polymerase ribozyme can be used to label the 3′ end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.