Development toxicity and cytotoxicity of pyroxsulam on embryos and adults of zebrafish (Danio rerio). (15th February 2023)

• Record Type: Journal Article
• Authors: Wang, Xiaole; Zhang, Jingwen; Lu, Chengbo; Liu, Yu; Yang, Xiao; Hou, Kaixuan; Du, Zhongkun; Li, Bing; Juhasz, Albert; Zhu, Lusheng
• Is Part Of: Environmental pollution. Volume 319(2023)
• Publication Date: 2023-02-15
• Subjects: Apoptosis -- DNA damage -- Integrated biomarker response -- Lipid peroxidation -- Oxidative stress; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2023.121040
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Pyroxsulam is a typical triazolopyrimidine sulfonamide herbicide, which plays a vital role in weed control for wheat production. Previous studies on pyroxsulam have mainly focused on weed resistance, control effects, and soil microorganisms, however, research on aquatic ecotoxicity is lacking. This study evaluated the toxicity of pyroxsulam in zebrafish embryos (120 h) and adults (14 and 28 d). Chronic exposure to pyroxsulam (0.2, 2, and 10 μg L −1 ) reduced reactive oxygen species (ROS) content in embryos but increased ROS content in adults. Pyroxsulam caused lipid peroxidation and DNA damage in embryos and adults. The expression of p53, bcl-2/bax, and caspase 3/8/9 indicated that pyroxsulam inhibited apoptosis in embryos but induced apoptosis in adults. By calculating integrated biomarker response, apoptosis was more readily affected than oxidative damage in embryos and adults. The toxicity of pyroxsulam increased with increasing concentration, however, with increasing exposure time, the toxicity of pyroxsulam to adults decreased. Pyroxsulam exerted toxic effects on zebrafish at different life stages (embryos and adults), and different stages had different toxicity. These results indicate that in future studies on the toxicity of pyroxsulam to zebrafish, different life stages (embryos and adults) need to be studied. The present study evaluated the toxicity of environmentally relevant concentrations of pyroxsulam to zebrafish embryos and adults, providing worthy data for assessing its effects on aquatic ecosystems. Graphical abstract: Image 1 Highlights: Pyroxsulam caused lipid peroxidation and DNA damage in embryos and adults. Pyroxsulam inhibited apoptosis in embryos but induced apoptosis in adults. Apoptosis was more readily affected than oxidative damage in embryos and adults. With increasing exposure time, the toxicity of pyroxsulam to adults decreased. The toxicity of pyroxsulam in adults at 28 d was different from that in embryos.