MiRNA-15/IL-10Rα axis promotes Kabasura Kudineer (Indian traditional Siddha formulation) induced immunomodulation by suppressing oxidative stress. (6th April 2023)

• Record Type: Journal Article
• Authors: Behera, Jyotirmaya; Pitchiah Kumar, M.; Ireen Femela, A.; Senguttuvan, Govindan; Ramasamy, M.S.
• Is Part Of: Journal of ethnopharmacology. Volume 305(2023)
• Publication Date: 2023-04-06
• Subjects: miRNA regulation -- Oxidative damage -- Immunomodulation -- IL-10Rα-3′ UTR -- Anti-inflammation; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2022.116032
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Kabasura Kudineer (KK), the traditional Indian medicine of Siddha, effectively manages common respiratory symptoms such as flu, cold, and fever. However, there is no evidence of the immunomodulatory capacity of KK in the cultured Jurkat T-lymphocytes under the LPS insult studied. Aim of the study: Assess the effect of the traditional Indian medicine of Siddha, Kabasura Kudineer (KK) on immunomodulation by suppressing oxidative damage in cultured Jurkat T cells in vitro . The miRNA activity on anti-inflammatory gene receptors and cellular nitric oxide levels also was studied. Materials and methods: Jurkat T cells were exposed to LPS treatment in the presence or absence of KK. Cell viability and nitric oxide (NO) were measured with MTT and Griess assay. Cellular antioxidant systems (glutathione and SOD) were determined using glutathione and SOD assay. Lipid peroxidation was measured using an MDA assay. MiRNA-15a-5p expression was performed using microRNA qPCR Assays. Both inflammatory and anti-inflammatory genes (IL-6, IL-1, IL-10, IL-13) were performed using a qPCR and ELISA assay. Results: The data showed that reduced cell proliferation and exaggerated NO production was observed in LPS treated condition compared to the control condition. Further, LPS treatment increased lipid peroxidation and reduced antioxidant enzyme activities (SOD and glutathione) in cultured Jurkat T cells. However, treatment with KK or N-acetyl cysteine (NAC; antioxidant) treatment mitigates the above effect. Mechanistically, LPS-induced oxidative stress upregulated miR- 15-5p expression and suppressed IL-10 Receptor alpha (IL-10Rα) by binding to its 3′-UTR region. The deregulated expression of IL-10Rα expression leads to increased IL-6 and IL-1β expression in LPS-induced Jurkat T cells; however, treatment with KK or NAC reversed the above effects. Conclusion: Collectively, our study revealed the previously undefined mechanistic role of Kabasura Kudineer (KK) that alleviates the LPS-induced oxidative damage associated with inflammation by inhibiting the miRNA-15-5p/IL-10Rα axis. Graphical abstract: Image 1 Highlights: LPS-induced Jurkat T-lymphocyte dysfunction is mediated by reduced NO production. Kabasura Kudineer treatment prevents LPS induced reduced NO production and improves the cellular redox homeostasis. Kabasura Kudineer prevents LPS accentuated Jurkat T-lymphocytes inflammation and suppression of IL-10Rα expression via miRNA-15/IL-10RA Axis. Kabasura Kudineer regulates immune modulation by upregulating anti-inflammatory genes (IL-10, IL-13) expression.