Microalgal extract as bio-coating to enhance biofilm growth of marine microalgae on microporous membranes. (February 2023)

• Record Type: Journal Article
• Authors: Tong, C.Y.; Chua, M.X.; Tan, Win Hung; Derek, C.J.C.
• Is Part Of: Chemosphere. Volume 315(2023)
• Publication Date: 2023-02
• Subjects: Biocoating -- Biofilm -- Cell adhesion -- Extracellular polymeric substances -- Microalgae; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.137712
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Microalgal biofilm is a popular platform for algal production, nutrient removal and carbon capture; however, it suffers from significant biofilm exfoliation under shear force exposure. Hence, a biologically-safe coating made up of algal extracellular polymeric substances (EPS) was utilized to secure the biofilm cell retention and cell loading on commercial microporous membrane (polyvinylidene fluoride), making the surfaces more hydrophobic (contact angle increase up to 12°). Results demonstrated that initial cell adhesion of three marine microalgae ( Amphora coffeaeformis, Cylindrotheca fusiformis and Navicula incerta ) was enhanced by at least 1.3 times higher than that of pristine control within only seven days with minimized biofilm exfoliation issue due to uniform distribution of sticky transparent exopolymer particles. Bounded extracellular polysaccharide gathered was approximately 23% higher on EPS-coated membranes to improve the biofilm's hydraulic resistance, whereas bounded extracellular protein would only be substantially elevated after the attached cells re-accommodate themselves onto the EPS pre-coating of themselves. In accounting the rises of hydrophobic protein content, biofilm was believed to be more stabilized, presumably via hydrophobic interactions. EPS biocoating would generate a groundswell of interest for bioprocess intensifications though there are lots of inherent technical and molecular challenges to be further investigated in future. Graphical abstract: Image 1 Highlights: EPS-coating modified microporous membranes enhanced microalgal cell adhesion. Biofilm cells secreted more polysaccharide to promote hydraulic resistance. EPS production was always species-specific with changing environmental conditions. EPS hydrophobicity was induced under EPS pre-coating.