Histopathological Changes in Liver and Heart Tissue Associated with Experimental Ultraviolet Radiation A and B Exposure on Wistar Albino Rats. (28th June 2022)

• Record Type: Journal Article
• Authors: Keskin, Seda; Acikgoz, Eda; Ertürk, Fatma Yilmaz; Ragbetli, Murat Cetin; Ozkol, Halil
• Is Part Of: Photochemistry and photobiology. Volume 99:Number 1(2023)
• Page Start: 132
• Page End: 136
• Publication Date: 2022-06-28
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.13664
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: This study aims to evaluate the influences of ultraviolet radiation A and B ( UVA + B) exposure on the liver and heart organs of albino rats. Female Wistar Albino rats, whose hair of the dorsal skin was shaved, were exposed to a combined UVA + B radiation for 2 h/day, for 4 weeks in order to be compared with the control group. Histopathological findings in vital organs (liver and heart) were evaluated. Tissues were fixed in 10% buffered formalin (pH = 7.2) and embedded in paraffin. The histopathological findings were examined on the H&E stained sections with light microscopy. The results show that the liver and the heart were injured in the UVA + B group. Liver tissue in the UVA + B group showed minimal vacuolation, enlargement of hepatocytes and bile duct proliferation, and the heart tissue showed hibernomas; uniform large cells resembling brown fat with coarsely granular to multivacuolated cytoplasm that is eosinophilic or pale with a small central nucleus. The number of hibernoma cases was significantly higher in the UVA + B group compared with the control group ( P = 0.021). The control group showed normal liver and heart histology with normal adipose tissue in the pericardium. As a result, UVA + B exposure has toxic effects, especially on the liver and the heart of Wistar albino rats. UV radiation may cause such adverse effects in humans. Therefore, protection against the harmful effects of UV radiation is of significant importance for skin and organs. Abstract : Before the UVA + B irradiation, the hair was shaved using electric clippers. Wistar albino rats were exposed to a combined UVA + B radiation 2 h/day for 1 month. Tissue samples of each group were fixed in 10% phosphate‐buffered formalin for morphological investigation. Liver and heart tissue sections were stained with H&E for histopathological assessment with light microscopy.