Recent advances in development of cell-free protein synthesis systems for fast and efficient production of recombinant proteins. Issue 17 (31st July 2018)

• Record Type: Journal Article
• Authors: Lee, Kyung-Ho; Kim, Dong-Myung
• Is Part Of: FEMS microbiology letters. Volume 365:Issue 17(2018:Sep.)
• Publication Date: 2018-07-31
• Subjects: cell-free expression -- recombinant protein production -- translation machinery -- cell extract -- ATP supply -- high-throughput; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fny174
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to the lack of physical barriers separating the protein synthesis machinery from the surrounding environment, a cell-free protein synthesis reaction mixture can be directly programmed using diverse genetic material for the instant production of recombinant proteins without complicated cloning procedures. However, a number of issues must be addressed for this technology to be widely accepted as an alternative platform for protein production, including quality-control of translation machinery preparations, and high reagent cost. This review describes recent efforts to make cell-free protein synthesis more affordable and more easily accessible for generic applications. Abstract : This review article surveys recent advances in cell-free protein synthesis technology.