The Effects of Acute Ammonia Exposure on the Immune Response of Juvenile Freshwater Prawn, Macrobrachium Nipponense. Issue 1 (1st January 2015)

• Record Type: Journal Article
• Authors: Zhang, Wenyi; Jiang, Qichen; Liu, Xiaoqian; Pan, Dongmei; Yang, Yuanhao; Yang, Jiaxin
• Is Part Of: Journal of crustacean biology. Volume 35:Issue 1(2015)
• Page Start: 76
• Page End: 80
• Publication Date: 2015-01-01
• Subjects: ammonia -- immune response -- LC50 values -- Macrobrachium nipponense; Crustacea -- Periodicals; Crustacés -- Périodiques; Crustacea; Periodicals; 595.305
• DOI: 10.1163/1937240X-00002292
• Languages: English
• ISSNs: 0278-0372

Abstract

Abstract: The effects of acute ammonia exposure on juvenile Macrobrachium nipponense (De Haan, 1849) were evaluated. The ${\rm{L}}{{\rm{C}}_{{\rm{50}}}}$ values and 95% confident intervals for total ammonia nitrogen (TAN) after 24, 48, 72 and 96 hours' exposure were 139.78 (108.77-179.63), 84.17 (50.40-127.87), 64.07 (44.78-91.66) and $40.42\, (30.46{\text{-}}53.62) \, {\rm{mg/l}}$ . Then a 96-hour acute toxicity experiment was conducted in five concentrations of TAN (0, 5, 10, 15 and $20 \, {\rm{mg/l}}$ ). The whole-body activities of alkaline phosphatase (AKP), acid phosphatase (ACP), superoxide dismutase (SOD), catalase (CAT), and the concentration of malonaldehyde (MDA) were measured. The results demonstrate that the concentrations used in this study induce the immune response and that damage may be accompanied by a depression of the antioxidant defense system and the accumulation of malonaldehyde (MDA). These results suggest that changes in whole-body immune parameters of juvenile prawns are effective to characterize overall prawn health and environmental conditions.