Compositional Assessment of Human Tracheal Cartilage by Infrared Spectroscopy. (16th January 2018)

• Record Type: Journal Article
• Authors: Linkov, Gary; Hanifi, Arash; Yousefi, Farzad; Tint, Derrick; Bolla, Sudheer; Marchetti, Nathanial; Soliman, Ahmed M. S.; Pleshko, Nancy
• Is Part Of: Otolaryngology--head and neck surgery. Volume 158:Number 4(2018)
• Page Start: 688
• Page End: 694
• Publication Date: 2018-01-16
• Subjects: NIR -- FTIR -- infrared spectroscopy -- trachea -- cartilage -- tissue engineering -- collagen; Head -- Surgery -- Periodicals; Neck -- Surgery -- Periodicals; Otolaryngology -- Periodicals; 617.51
• DOI: 10.1177/0194599817752310
• Languages: English
• ISSNs: 0194-5998

Abstract

Abstract : Objectives: To assess the potential of infrared fiber‐optic spectroscopy to evaluate the compositional properties of human tracheal cartilage. Study Design: Laboratory‐based study. Methods: Twenty human cadaveric distal tracheas were harvested (age range 20‐78 years; 6 females, 14 males) for compositional analysis. Histologic staining, Fourier transform infrared imaging spectroscopy data on collagen and proteoglycan (PG) content, and near‐infrared (NIR) fiber‐optic probe spectroscopic data that reflect protein and water content were evaluated. NIR fiber‐optic probe data were also obtained from the proximal trachea in 4 human cadavers (age range 51‐65 years; 2 females, 2 males) in situ for comparison to distal trachea spectral data. Results: In the distal trachea cohort, the spectroscopic‐determined ratio of PG/amide I, indicative of the relative amount of PG, was significantly higher in the tissues from the younger group compared to the older group (0.37 ± 0.08 vs 0.32 ± 0.05, P =. 05). A principal component analysis of the NIR spectral data enabled separation of spectra based on tracheal location, likely due to differences in both protein and water content. The NIR‐determined water content based on the 5200‐cm −1 peak was significantly higher in the distal trachea compared to the proximal trachea ( P <. 001). Conclusions: Establishment of normative compositional values and further elucidating differences between the segments of trachea will enable more directed research toward appropriate compositional end points in regenerative medicine for tracheal repair.