Controllable Synthesis of BiOCl with Z‐Scheme (001)/(110) Facet Homojunction and their Photocatalytic Killing Effect on HePG2 Cells in vitro. (6th June 2022)

• Record Type: Journal Article
• Authors: Dong, Wei; Liang, Erli; Zhang, Qiong; Li, Rui; Wang, Yunfang; Liu, Jianxin; Zhang, Xiaochao; Wang, Yawen; Fan, Caimei
• Is Part Of: Photochemistry and photobiology. Volume 99:Number 1(2023)
• Page Start: 83
• Page End: 91
• Publication Date: 2022-06-06
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.13657
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: In this study, a set of BiOCl with controllable ratios of (001) and (110) facets was prepared by adjusting the content of diethylene glycol (DEG) during the preparation process. The degradation experiment of bisphenol A (BPA) shows that under simulated sunlight, when the ratio of (001) to (110) is 0.61, BiOCl (BOC‐2) has the best degradation activity, which can degrade 96.2% BPA within 20 min. After theoretical calculations and experimental characterization, a Z‐scheme (001)/(110) facet homojunction is proposed. Then, three typical samples were selected to test the biological toxicity of HepG2 cells and the activity of killing HepG2 cells under ultraviolet light conditions. Studies have found that exposed facets play a more important role in the biotoxicity of BiOCl to cells; with a (001)/(110) ratio of 0.61, BOC‐2 exhibits excellent endocytosis and phototoxicity but no obvious dark cytotoxicity, while with a (001)/(110) ratio of 0.15, BiOCl (BOC‐4) has poor endocytosis and strong cytotoxicity under dark conditions. Through reactive oxygen species (ROS) and lactate dehydrogenase (LDH) assay detection, the process of photocatalytic killing cells of BOC‐2 more looks like an apoptosis mechanism, while BOC‐4 mainly causes cell necrosis. Abstract : The photocatalytic effect of BOC‐2 on cells is mainly caused by apoptosis, and BOC‐4 is mainly caused by necrotic damage to cells. BOC‐2 suggests a higher photocatalytic killing effect on HepG2 cells due to its highly effective cellular uptake and higher ROS production.