Coupling of replisome movement with nucleosome dynamics can contribute to the parent–daughter information transfer. Issue 10 (21st March 2018)

• Record Type: Journal Article
• Authors: Bameta, Tripti; Das, Dibyendu; Padinhateeri, Ranjith
• Is Part Of: Nucleic acids research. Volume 46:Issue 10(2018)
• Page Start: 4991
• Page End: 5000
• Publication Date: 2018-03-21
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gky207
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Positioning of nucleosomes along the genomic DNA is crucial for many cellular processes that include gene regulation and higher order packaging of chromatin. The question of how nucleosome-positioning information from a parent chromatin gets transferred to the daughter chromatin is highly intriguing. Accounting for experimentally known coupling between replisome movement and nucleosome dynamics, we propose a model that can obtain de novo nucleosome assembly similar to what is observed in recent experiments. Simulating nucleosome dynamics during replication, we argue that short pausing of the replication fork, associated with nucleosome disassembly, can be a event crucial for communicating nucleosome positioning information from parent to daughter. We show that the interplay of timescales between nucleosome disassembly (τ p ) at the replication fork and nucleosome sliding behind the fork (τ s ) can give rise to a rich 'phase diagram' having different inherited patterns of nucleosome organization. Our model predicts that only when τ p ≥ τ s the daughter chromatin can inherit nucleosome positioning of the parent.