A newly isolated Enterobacter sp. strain produces 2, 3-butanediol during its cultivation on low-cost carbohydrate-based substrates. Issue 1 (26th November 2018)

• Record Type: Journal Article
• Authors: Palaiogeorgou, Anastasia Marina; Papanikolaou, Seraphim; de Castro, Aline Machado; Freire, Denise Maria Guimarães; Kookos, Ioannis K; Koutinas, Apostolis A
• Is Part Of: FEMS microbiology letters. Volume 366:Issue 1(2019)
• Publication Date: 2018-11-26
• Subjects: acetoin -- 2, 3-butanediol -- Enterobacter sp -- molasses -- sucrose; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fny280
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: 2, 3-Butanediol (BDO) is an important platform chemical with a wide range of applications in various industries. In the present study, a newly isolated wild Enterobacter sp. strain (FMCC-208) was evaluated towards its ability to produce BDO on media composed of sugars derived from sucrose refinery plant. Optimum values of temperature and pH as well as substrate inhibition were determined through batch experiments. The ability of the strain to convert various monosaccharides was also investigated. Maximum BDO concentrations of 90.3 and 10 g l – 1 of acetoin were obtained during a fed-batch bioreactor experiment with cane molasses and sucrose employed as substrates. A high volumetric productivity was noted in a fed-batch experiment using molasses and sucrose as carbon sources at T = 37°C, in which 73.0 g l – 1 of BDO together with 12.4 g l – 1 of acetoin was produced where 1.15 g l – 1 h – 1 of diol/acetoin was produced. In previously pasteurized media, 70.0 g l – 1 of BDO and 5.0 g l – 1 of acetoin were produced (yield = 0.39 g g – 1 ). Finally, besides BDO production, growth on molasses was accompanied by non-negligible decolorization (25–35%) of the residue. Therefore, the strain is a promising candidate for the conversion of sucrose-based materials into BDO. Abstract : A new food-derived and potentially non-pathogenic wild-type strain of Enterobacter sp. produces remarkable 2, 3-butanediol quantities from low-cost sugars employed as substrates.