Viewing pre-60S maturation at a minute's timescale. Issue 6 (27th December 2017)
- Record Type:
- Journal Article
- Title:
- Viewing pre-60S maturation at a minute's timescale. Issue 6 (27th December 2017)
- Main Title:
- Viewing pre-60S maturation at a minute's timescale
- Authors:
- Zisser, Gertrude
Ohmayer, Uli
Mauerhofer, Christina
Mitterer, Valentin
Klein, Isabella
Rechberger, Gerald N
Wolinski, Heimo
Prattes, Michael
Pertschy, Brigitte
Milkereit, Philipp
Bergler, Helmut - Abstract:
- Abstract: The formation of ribosomal subunits is a highly dynamic process that is initiated in the nucleus and involves more than 200 trans -acting factors, some of which accompany the pre-ribosomes into the cytoplasm and have to be recycled into the nucleus. The inhibitor diazaborine prevents cytoplasmic release and recycling of shuttling pre-60S maturation factors by inhibiting the AAA-ATPase Drg1. The failure to recycle these proteins results in their depletion in the nucleolus and halts the pathway at an early maturation step. Here, we made use of the fast onset of inhibition by diazaborine to chase the maturation path in real-time from 27SA2 pre-rRNA containing pre-ribosomes localized in the nucleolus up to nearly mature 60S subunits shortly after their export into the cytoplasm. This allows for the first time to put protein assembly and disassembly reactions as well as pre-rRNA processing into a chronological context unraveling temporal and functional linkages during ribosome maturation.
- Is Part Of:
- Nucleic acids research. Volume 46:Issue 6(2018)
- Journal:
- Nucleic acids research
- Issue:
- Volume 46:Issue 6(2018)
- Issue Display:
- Volume 46, Issue 6 (2018)
- Year:
- 2018
- Volume:
- 46
- Issue:
- 6
- Issue Sort Value:
- 2018-0046-0006-0000
- Page Start:
- 3140
- Page End:
- 3151
- Publication Date:
- 2017-12-27
- Subjects:
- Nucleic acids -- Periodicals
Molecular biology -- Periodicals
572.805 - Journal URLs:
- http://nar.oxfordjournals.org/ ↗
http://www.ncbi.nlm.nih.gov/pmc/journals/4 ↗
http://ukcatalogue.oup.com/ ↗
http://firstsearch.oclc.org ↗ - DOI:
- 10.1093/nar/gkx1293 ↗
- Languages:
- English
- ISSNs:
- 0305-1048
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6183.850000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 25164.xml