Purification and Molecular Cloning of Hemocyanin from Fenneropenaeus Merguiensis (): Response to Vibrio Harveyi Exposure. Issue 5 (1st September 2015)

• Record Type: Journal Article
• Authors: Jiewkok, Acharee; Tsukimura, Brian; Utarabhand, Prapaporn
• Is Part Of: Journal of crustacean biology. Volume 35:Issue 5(2015)
• Page Start: 659
• Page End: 669
• Publication Date: 2015-09-01
• Subjects: banana shrimp -- Fenneropenaeus merguiensis -- hemocyanin -- phenoloxidase -- shrimp immunity; Crustacea -- Periodicals; Crustacés -- Périodiques; Crustacea; Periodicals; 595.305
• DOI: 10.1163/1937240X-00002356
• Languages: English
• ISSNs: 0278-0372

Abstract

Abstract: Hemocyanin (HC) was purified from hemolymph of Fenneropenaeus merguiensis (de Man, 1888 ). Purified HC showed one band in non-denaturing PAGE with a molecular mass of 457 kDa, determined by gel filtration. Using an ELISA, HC content was 85.6% of total hemolymph protein. HC's hexamer form contained no phenoloxidase activity under trypsin or SDS activation. FmHC, cloned from F. merguiensis, had a full-length cDNA comprised of 2128 bp with an open reading frame encoding a peptide of 661 amino acids with two Cu-binding sites. Expression of FmHC was found only in the hepatopancreas where it was up-regulated after exposure to Vibrio harveyi injection into shrimp and with tissue incubations. HC protein levels in the hemolymph of bacterial injected shrimp also increased. FmHC expression was also up-regulated after shrimp were challenged with a lipopolysaccharide, which is a surface component of gram-negative bacteria. These results indicate that HC is inducible and up-regulated in response to pathogenic challenge, confirming that HC participates in the innate immune response in F. merguiensis .