Enhanced production and immobilization of phytase from Aspergillus oryzae: a safe and ideal food supplement for improving nutrition. (26th December 2022)

• Record Type: Journal Article
• Authors: , Pragya; Sharma, Krishna Kant; Kumar, Sandeep; , Manisha; Singh, Davender; Kumar, Vijay; Singh, Bijender
• Is Part Of: Letters in applied microbiology. Volume 74:Number 3(2022)
• Publication Date: 2022-12-26
• Subjects: Phytase -- optimization -- Immobilization -- Stability -- Safety assessment -- Nutrition; Microbiology -- Periodicals; 660.62
• DOI: 10.1093/lambio/ovac077
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: Microbial phytases are potentially excellent candidates for eliminating anti-nutrient i.e. phytic acid, due to hydrolysis of phospho-monoester linkages present in the phytic acid. An average 2.29-fold increase in phytase production was obtained after statistical optimization in solid-state fermentation. Aspergillus oryzae SBS50 phytase was immobilized on Ca-alginate matrix with an effectiveness of 53%. Immobilized-phytase retained more than 50% activity after recycling for five cycles and also displayed more stability in the presence of organic solvents, metal ions, and detergents as compared to free enzyme. Values of Km and Vmax of immobilized phytase were recorded as 0.66 mM and 666.6 nmol/sec, respectively. Immobilized phytase efficiently hydrolyzed the phytate contents in wheat and pearl millet flours exhibiting more than 70% catalytic activity even after three cycles. Phytase supplementation resulted in the improved nutritional quality of these flours. Furthermore, the safety assessment of the treated and untreated samples reveals the absence of any aflatoxin in the phytase produced by the mould. The results revealed the improved stability of phytase after immobilization and as a safe and significant additive for application in the food industry.