Domestication of Lambda Phage Genes into a Putative Third Type of Replicative Helicase Matchmaker. (27th June 2017)

• Record Type: Journal Article
• Authors: Brézellec, Pierre; Petit, Marie-Agnès; Pasek, Sophie; Vallet-Gely, Isabelle; Possoz, Christophe; Ferat, Jean-Luc
• Is Part Of: Genome biology and evolution. Volume 9:Number 6(2017:Jun.)
• Page Start: 1561
• Page End: 1566
• Publication Date: 2017-06-27
• Subjects: replicative helicase -- replication initiation -- viral gene domestication -- dnaC -- dciA -- lambda phage; Genomics -- Periodicals; Genes -- Periodicals; 572.8605
• DOI: 10.1093/gbe/evx111
• Languages: English
• ISSNs: 1759-6653

Abstract

Abstract: At the onset of the initiation of chromosome replication, bacterial replicative helicases are recruited and loaded on the DnaA- oriC nucleoprotein platform, assisted by proteins like DnaC/DnaI or DciA. Two orders of bacteria appear, however, to lack either of these factors, raising the question of the essentiality of these factors in bacteria. Through a phylogenomic approach, we identified a pair of genes that could have substituted for dciA . The two domesticated genes are specific of the dnaC / dnaI - and dciA -lacking organisms and apparently domesticated from lambdoid phage genes. They derive from λO and λP and were renamed dopC and dopE, respectively. DopE is expected to bring the replicative helicase to the bacterial origin of replication, while DopC might assist DopE in this function. The confirmation of the implication of DopCE in the handling of the replicative helicase at the onset of replication in these organisms would generalize to all bacteria and therefore to all living organisms the need for specific factors dedicated to this function.