A multivariate curve resolution‐alternating least squares (MCR‐ALS) technology assisted 1H‐NMR methodology for multi‐component quantitation of Trichosanthis Pericarpium injection. Issue 1 (24th October 2022)

• Record Type: Journal Article
• Authors: Yang, Jiayu; Li, Wenzhu; Ding, Jinguo; Dong, Ying; Xie, Xinyuan; Zhao, Fang; Pan, Jianyang; Qu, Haibin
• Is Part Of: Phytochemical analysis. Volume 34:Issue 1(2023)
• Page Start: 40
• Page End: 47
• Publication Date: 2022-10-24
• Subjects: 1H‐NMR -- multi‐component quantitation method -- multivariate curve resolution‐alternating least squares (MCR‐ALS) -- traditional Chinese medicine injection (TCMI) -- Trichosanthis Pericarpium injection (TPI); Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.3177
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Trichosanthis Pericarpium injection (TPI) is a traditional Chinese medicine preparation obtained from Trichosanthis Pericarpium by extraction, purification and sterilisation. It contains amino acids, alkaloids, nucleotides and other components. Existing quantitative methods only analyse a few components in injections, so this study intends to develop a method for comprehensive analysis of TPI components. Objective: To develop a method for quantification of components in TPI by multivariate curve resolution‐alternating least squares (MCR‐ALS) assisted proton nuclear magnetic resonance ( 1 H‐NMR). Methods: A 1 H‐NMR method was developed for the quantification of components in TPI. For components with independent signals, 3‐(trimethylsilyl) propionic‐2, 2, 3, 3‐ d 4 acid sodium salt (TSP) was used as an internal standard to calculate the component contents. For components with overlapping signals, the method of MCR‐ALS was used. Results: A total of 36 components were identified in TPI, of which 33 were quantified. Methodological validation results showed that the developed 1 H‐NMR method has good linearity, accuracy, precision, robustness and specificity. Conclusion: The use of 1 H‐NMR provides a reliable and universal method for the TPI components identification and quantification. Also, it can be used as a powerful tool for analysing the contents in a complex mixture as a quality control measure. Abstract : In this study, a method for quantifying 33 components in Trichosanthis Pericarpium injection (TPI) by proton nuclear magnetic resonance ( 1 H‐NMR) was proposed. The 1 H‐NMR provided a reliable and universal quantitative method for TPI components analysis. Also, it can be used as a powerful tool for analysing the contents in a complex mixture as a quality control measure.