Solicola gregarius gen. nov., sp. nov., a soil actinobacterium isolated after enhanced cultivation with Micrococcus luteus culture supernatant. Issue 1 (24th January 2023)

• Record Type: Journal Article
• Authors: Lopez Marin, Marco A.; Suman, Jachym; Jani, Kunal; Ulbrich, Pavel; Cajthaml, Tomas; Filipova, Alena; Pajer, Petr; Neumann-Schaal, Meina; Strejcek, Michal; Uhlik, Ondrej
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 1(2023)
• Publication Date: 2023-01-24
• Subjects: compost soil -- extracellular organic matter -- Nocardioidaceae -- resuscitation-promoting factor -- soil bacteria -- whole-genome average nucleotide identity; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005678
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An actinobacterial strain, designated A5X3R13 T, was isolated from a compost soil suspension supplemented with extracellular material from a Micrococcus luteus -culture supernatant. The strain was cultured on tenfold-diluted reasoner's 2A agar. The cells were ovoid-to-rod shaped, non-motile, Gram-stain-positive, oxidase-negative, catalase-positive and had a width of 0.5 µm and a length of 0.8–1.2 µm. The results of both 16S rRNA-based phylogenetic and whole-genome analyses indicate that A5X3R13 T forms a distinct lineage within the family Nocardioidaceae (order Propionibacteriales ). On the basis of the 16S rRNA gene sequence, A5X3R13 T was closely related to Aeromicrobium terrae CC-CFT486 T (96.2 %), Nocardioides iriomotensis IR27-S3 T (96.2 %), Nocardioides guangzhouensis 130 T (95.6 %), Marmoricola caldifontis YIM 730233 T (95.5 %), Aeromicrobium alkaliterrae KSL-107 T (95.4 %), Aeromicrobium choanae 9H-4 T (95.4 %), Aeromicrobium panaciterrae Gsoil 161 T (95.3 %), and Nocardioides jensenii NBRC 14755 T (95.2 %). The genome had a length of 4 915 757 bp, and its DNA G+C content was 68.5 mol %. The main fatty acids were 10-methyl C17 : 0, C16 : 0, C15 : 0, C18 : 0, C17 : 0 and iso-C16 : 0 . The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. MK-9(H4 ) was the predominant respiratory quinone. The peptidoglycan type was A3γ (A41.1) and contained alanine, glycine, glutamic acid and ll -diaminopimelic acid in a molar ratio of 1.2 : 0.9 : 1.0 : 0.8. On the basis of the results of the phylogenetic and phenotypic analyses and comparisons with other members of the family Nocardioidaceae, strain A5X3R13 T is proposed to represent a novel species within a novel genus, for which the name Solicola gregarius gen. nov., sp. nov. is proposed. The type strain is A5X3R13 T (=DSM 112953 T =NCCB 100840 T ).