Vibrio sinus sp. nov., a marine bacterium isolated from coastal seawater. Issue 1 (18th January 2023)

• Record Type: Journal Article
• Authors: Wang, Wei; Zhao, Wenbin; Cao, Bingbing; Liu, Ronghua; Cheng, Haojin; Tian, Peng; Xiao, Jiaguang; Xu, Ziqing; Wang, Xiaolei; Niu, Wentao
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 1(2023)
• Publication Date: 2023-01-18
• Subjects: Dongshan Bay -- surface seawater -- Vibrio sinus sp. nov; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005676
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram–straining–negative, facultatively anaerobic, motile by means of a polar flagellum and rod-shaped marine bacterium, designated S4M6 T, was isolated from surface seawater collected in Dongshan Bay (Fujian, PR China). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole genome data indicated that S4M6 T represented a member of the genus Vibrio . The closest phylogenetic relatives of S4M6 T were Vibrio marisflavi CGMCC 1.8994 T (97.8 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis LMG 25438 T (96.9 %), Vibrio gangliei SZDIS-1 T (96.2 %) and Vibrio aestivus M22 T (96.1 %). The growth of S4M6 T occurred at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum 5.0–7.0) and in the presence of 2–5 % (w/v) NaCl (optimum 3 %). The predominant fatty acids (>10 %) are C16 : 0, summed feature 3 (C16 : 1 ω7 c and/or C16 : 1 ω6c) and summed feature 8 (C18 : 1 ω7 c and/or C18 : 1 ω6 c ). The DNA G+C content of the assembled genomic sequences was 43.4 % for S4M6 T . Average nucleotide identity (ANI) values between S4M6 T and the reference species were lower than the threshold for species delineation (95–96 %); in silico DNA–DNA hybridization further indicated that S4M6 T had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4M6 T is proposed to represent a novel species of the genus Vibrio, for which the name Vibrio sinus sp. nov. is proposed. The type strain is S4M6 T (= KCTC 92312 T = MCCC 1K06167 T ).