Dynamics of Human Anelloviruses in Plasma and Clinical Outcomes Following Kidney Transplantation. Issue 2 (26th January 2023)

• Record Type: Journal Article
• Authors: Forqué, Lorena; Fernández-Ruiz, Mario; Albert, Eliseo; Giménez, Estela; Monzó, Carolina; Chaves, Javier; Redondo, Natalia; Rodríguez-Goncer, Isabel; Ruiz-Merlo, Tamara; Parra, Patricia; Andrés, Amado; Aguado, José María; Navarro, David
• Is Part Of: Transplantation. Volume 107:Issue 2(2023)
• Page Start: 511
• Page End: 520
• Publication Date: 2023-01-26
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; Transplantation immunology -- Periodicals; 617.95
• DOI: 10.1097/TP.0000000000004292
• Languages: English
• ISSNs: 0041-1337

Abstract

Abstract : Background: Torque teno virus, the major member of the genus Alphatorquevirus, is an emerging biomarker of the net state of immunosuppression after kidney transplantation. Genetic diversity constitutes a main feature of the Anelloviridae family, although its posttransplant dynamics and clinical correlates are largely unknown. Methods: The relative abundance of Alphatorquevirus, Betatorquevirus, and Gammatorquevirus genera was investigated by high-throughput sequencing in plasma specimens obtained at various points during the first posttransplant year (n = 91 recipients). Total loads of all members of the Anelloviridae family were also quantified by an "in-house" polymerase chain reaction assay targeting conserved DNA sequences (n = 195 recipients). In addition to viral kinetics, clinical study outcomes included serious infection, immunosuppression-related adverse event (opportunistic infection and cancer)‚ and acute rejection. Results: Alphatorquevirus DNA was detected in all patients at every point, with an increase from pretransplantation to month 1. A variable proportion of recipients had detectable Betatorquevirus and Gammatorquevirus at lower frequencies. At least 1 change in the predominant genus (mainly as early transition to Alphatorquevirus predominance) was shown in 35.6% of evaluable patients. Total anelloviruses DNA levels increased from baseline to month 1, to peak by month 3 and decrease thereafter, and were higher in patients treated with T-cell depleting agents. There was a significant albeit weak-to-moderate correlation between total anelloviruses and TTV DNA levels. No associations were found between the predominant Anelloviridae genus or total anelloviruses DNA levels and clinical outcomes. Conclusions: Our study provides novel insight into the evolution of the anellome after kidney transplantation. Abstract :