CgIL17-5 regulates the mRNA expressions of immune effectors through inducing the phosphorylation of CgMAPKs and the nuclear translocation of CgRel and CgAP-1 in the Pacific oyster Crassostrea gigas. (February 2022)

• Record Type: Journal Article
• Authors: Lv, Xiaoqian; Sun, Jiejie; Li, Yinan; Yang, Wenwen; Wang, Liyan; Leng, Jinyuan; Yan, Xiaoxue; Guo, Zhicheng; Yang, Qian; Wang, Lingling; Song, Linsheng
• Is Part Of: Developmental and comparative immunology. Volume 127(2022)
• Publication Date: 2022-02
• Subjects: Interleukin 17 -- Mitogen-activated protein kinase -- Activator protein-1 -- Rel -- Crassostrea gigas; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2021.104263
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interleukin-17 (IL-17) is a classic pro-inflammatory cytokine that plays an important role in the immune and inflammatory response. In the present study, the sequence feature of Cg IL17-5 and its function as a pro-inflammatory factor in inducing the mRNA expressions of downstream immune effectors were investigated in oyster Crassostrea gigas . There were two tightly folded alpha helixes and two pairs of antiparallel beta-pleated sheet in the amino acid sequence of Cg IL17-5. The mRNA transcripts of Cg IL17-5 were constitutively distributed in all the tested tissues, with the highest level in haemocytes. The mRNA expression level of Cg IL17-5 in haemocytes increased significantly at 24 h after Vibrio splendidus stimulation. Cg IL17-5 protein was mainly detected in granulocytes which were the main immunocompetent haemocytes in C. gigas . The phosphorylation of mitogen-activated protein kinases ( Cg JNK, Cg ERK and Cg P38) and nuclear translocation of the transcription factors ( Cg Rel and Cg AP-1) in haemocytes were induced after the oysters received an injection of recombinant Cg IL17-5 for 2 h. The mRNA expression levels of Cg IL-17s, Cg TNF-1, Cg defh1 and Cg defh2 increased significantly in haemocytes. At the same time, obvious branchial swelling and cilium shedding in gills were observed at 24 h after the oysters received an injection of r Cg IL17-5. All the results collectively suggested that Cg IL17-5 promoted the activation of Cg MAPKs and the nuclear translocation of Cg Rel and Cg AP-1 to promote the mRNA expressions of cytokines and antibacterial peptides. Highlights: 1. Cg IL17-5 induced the phosphorylation of Cg JNK, Cg ERK and Cg P38 in haemocytes. 2. Cg IL17-5 promoted the nuclear translocation of Cg Rel and Cg AP-1 in haemocytes. 3. Cg IL17-5 regulated the production of cytokines and antimicrobial peptides.