Metagenomics: novel enzymes from non-culturable microbes. Issue 21 (4th October 2017)

• Record Type: Journal Article
• Authors: Berini, Francesca; Casciello, Carmine; Marcone, Giorgia Letizia; Marinelli, Flavia
• Is Part Of: FEMS microbiology letters. Volume 364:Issue 21(2017)
• Publication Date: 2017-10-04
• Subjects: metagenomics -- environmental libraries -- screening -- heterologous expression -- industrial enzymes; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnx211
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely untapped by using traditional isolation and cultivation methods. Metagenomics, which is culture independent, represents a powerful tool for discovering novel enzymes from unculturable microorganisms. Herein, we summarize the variety of approaches adopted for mining environmental DNA and, based on a systematic literature review, we provide a comprehensive list of 332 industrially relevant enzymes discovered from metagenomes within the last three years. Abstract : Metagenomics is a powerful tool for identifying novel microbial biocatalysts.