CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement. (17th April 2018)

• Record Type: Journal Article
• Authors: Hussain, Babar; Lucas, Stuart James; Budak, Hikmet
• Is Part Of: Briefings in functional genomics. Volume 17:Number 5(2018:Sep.)
• Page Start: 319
• Page End: 328
• Publication Date: 2018-04-17
• Subjects: CRISPR/Cas9 -- crop improvement -- food nutrition -- climate-resilient crops -- plant defense response; Genomics -- Methodology -- Periodicals; Genomics -- Technological innovations -- Periodicals; 572.86072
• DOI: 10.1093/bfgp/ely016
• Languages: English
• ISSNs: 2041-2649

Abstract

Abstract: The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple, robust, powerful tool for targeted gene mutagenesis, knockout and knock-in/replacement, as well as transcriptional regulation. Here, we review the working principles, components and potential modifications of CRISPR/Cas9 for efficient single and multiplex gene editing in plants. We also describe recent work that has used CRISPR/Cas9 to improve economically important traits in crop plants. Although the apparent ease of CRISPR/Cas9-mediated editing may make it appear as though scientists are merely playing with plant genomes, the combined power of CRISPR/Cas9 has enabled vital research to be completed in the battle toward optimization and adaptation of crop species, permitting crucial advances to be achieved in crop improvement.