Daily Biopsy Diagnosis in Surgical Pathology: Concordance Between Light Microscopy and Whole-Slide Imaging in Real-Life Conditions. Issue 4 (14th February 2018)

• Record Type: Journal Article
• Authors: Villa, Irène; Mathieu, Marie-Christine; Bosq, Jacques; Auperin, Anne; Pomerol, Jean-François; Lacroix-Triki, Magali; Scoazec, Jean-Yves; Dartigues, Peggy
• Is Part Of: American journal of clinical pathology. Volume 149:Issue 4(2018)
• Page Start: 344
• Page End: 351
• Publication Date: 2018-02-14
• Subjects: Digital pathology -- Whole-slide imaging -- Biopsy pathology -- Diagnostic concordance; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqx161
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: The current challenge for the various digital whole-slide imaging (WSI) systems is to be definitively validated for diagnostic purposes. We designed a concordance study between glass slide and digital slide diagnosis in real-life conditions, coupled with an ergonomic study. Methods: Three senior pathologists evaluated, first in glass slides and then in digital slides, 119 biopsy cases, including 749 slides, with 332 H&E saffron stains and 417 additional techniques, mainly immunohistochemistry. Results: All digital slides, including specially stained slides, were interpretable. Concordance between glass slides and digital slides was observed in 87.4% of cases. Minor discordances were observed in 12 (10.1%) cases and major discordances, with therapeutic impact, in three (2.5%), including one related to WSI. The satisfaction of participants was high and increased with time. Conclusions: Our study confirms the feasibility and accuracy of WSI diagnosis, even for cases having multiple samples and requiring special staining techniques, such as immunohistochemistry and in situ hybridization.