Bladder Smooth Muscle Cells Interaction and Proliferation on PCL/PLLA Electrospun Nanofibrous Scaffold. Issue 2 (February 2013)

• Record Type: Journal Article
• Authors: Shakhssalim, Nasser; Rasouli, Javad; Moghadasali, Reza; Aghdas, Farzaneh Sharifi; Naji, Mohammad; Soleimani, Masoud
• Is Part Of: International journal of artificial organs. Volume 36:Issue 2(2013)
• Page Start: 113
• Page End: 120
• Publication Date: 2013-02
• Subjects: Urinary bladder -- Smooth muscle cells -- Nanofibers -- Materials testing; Artificial organs -- Periodicals; 617.956
• DOI: 10.5301/ijao.5000175
• Languages: English
• ISSNs: 0391-3988

Abstract

Purpose: Numerous synthetic materials have been used for the bladder reconstruction; of which, nano-structured scaffolds are used as relevant implant to the bladder tissue-engineering. The aim of this study was to investigate the capacity of Poly ∊-caprolactone/poly-L-lactide acid (PCL/PLLA) nanofibrous scaffold, in supporting the maintenance and attachment of the human bladder smooth muscle cells (BdSMCs). Methods: In this study, BdSMCs were isolated by enzymatic digestion method. Then, cells were seeded on PCL/PLLA nanofibrous scaffolds. Thereafter, cell attachment and expansion were analyzed by Hematoxylin and Eosin staining (H&E), immunohistochemistry, and scanning electron microscopy (SEM). 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay confirmed that the nanostructured scaffold supported and maintained normal cell viability without inducing cytotoxic events. Results: H&E staining, immunohistochemistry, and SEM showed that BdSMCs were attached and expanded on PCL/PLLA nanofibrous scaffolds after 14 days. Cell viability of BdSMCs on PCL/PLLA nanofibrous scaffolds increased during 14 days. Conclusion: Our results showed that the novel porous nanofibrous electrospun scaffold is a biocompatible structure for attachment and adhesion of BdSMCs. However, there is not enough information on the stimulating effect of this nanofiber on the cells. Therefore, further in-vivo studies seem required to confirm such a nanofiber to be used in the bladder tissue-engineering.