Genetic variants in microRNAs are associated with cervical cancer risk. (10th March 2019)

• Record Type: Journal Article
• Authors: Wang, Shizhi; Zhu, Haixia; Ding, Bo; Feng, Xinrui; Zhao, Wenxuan; Cui, Mengjing; Xu, Yuling; Shi, Minxin; Chen, Jian; Jin, Hua
• Is Part Of: Mutagenesis. Volume 34:Number 2(2019)
• Page Start: 127
• Page End: 133
• Publication Date: 2019-03-10
• Subjects: Mutagenesis -- Periodicals; Mutagenicity Tests -- Periodicals; Mutagens -- Periodicals; Mutagenesis; Periodicals; 576.542
• DOI: 10.1093/mutage/gez005
• Languages: English
• ISSNs: 0267-8357

Abstract

Abstract: Because genetic variants in microRNAs (miRNAs) or their surrounding regions can alter miRNA processing, expression and final biological function, we investigated whether miRNA single-nucleotide polymorphisms (SNPs) are associated with cervical cancer (CC) susceptibility. Common miRNA SNPs (i.e. miR-146a rs2910164, miR-149 rs2292832, miR-196a2 rs11614913, miR-499 rs3746444, miR-605 rs2043556 and miR-618 rs2682818) were genotyped in the 954 patients and 1339 controls. The results showed that the miR-149 rs2292832 TC/CC genotypes were associated with a 21% increased risk of CC compared with the TT genotype [odds ratio (OR) = 1.21, 95% confidence interval (CI) = 1.00–1.47]. The association was more prominent among the subjects with age ≤ 48 years (OR = 1.55, 95% CI = 1.16–2.06), having history of abortion (OR = 1.44, 95% CI = 1.12–1.86), premenopausal status (OR = 1.41, 95% CI = 1.08–1.85) and patients with clinical stage II of CC (OR = 1.43, 95% CI = 1.08–1.90). The expression plasmids containing the pre-miR-149 sequence with C allele of rs2292832 transcribed higher amount of mature miR-149-5p/3p than these with T allele in the HeLa and SiHa cells. Therefore, the rs2292832 polymorphism might influence CC susceptibility through modulation of the procession of pre-miR-149 to mature miRNAs.