Inhibition of SIRT1 by miR-138-5p provides a mechanism for inhibiting osteoblast proliferation and promoting apoptosis under simulated microgravity. (February 2023)

• Record Type: Journal Article
• Authors: Xu, Liqun; Zhang, Xiaoyan; Li, Gaozhi; Zhang, Lijun; Zhang, Shu; Shi, Fei; Hu, Zebing
• Is Part Of: Life sciences in space research. Volume 36(2023)
• Page Start: 59
• Page End: 69
• Publication Date: 2023-02
• Subjects: MiR-138-5p -- SIRT1 -- Microgravity -- Proliferation -- Apoptosis; Space biology -- Periodicals; 571.0919
• DOI: 10.1016/j.lssr.2022.08.001
• Languages: English
• ISSNs: 2214-5524

Abstract

Highlights: Microgravity disrupts the balance of bone remodeling. MiR-138-5p was a mechanosensitive miRNA and inhibited osteoblast proliferation and induced apoptosis under simulated microgravity. SIRT1 was downregulated and negatively correlated with the expression of miR-138-5p under simulated microgravity. MiR-138-5p inhibited osteoblast proliferation and promoted osteoblast apoptosis by targeting SIRT1. Abstract: Microgravity can inhibit osteoblast proliferation and promote apoptosis, which is related to a reduction in mechanical stress on the bones and results in disuse osteoporosis, but the detailed mechanism is still unclear. In this study, we first demonstrated that miR-138-5p was upregulated, inhibited osteoblast proliferation and induced osteoblast apoptosis under simulated microgravity. Moreover, miR-138-5p silencing partially mitigated the effects of proliferation and apoptosis of MC3T3-E1 cells. Our study further showed that sirtuin 1 (SIRT1) was downregulated and negatively correlated with the expression of miR-138-5p under simulated microgravity, which indicated that miR-138-5p inhibited osteoblast proliferation and promoted osteoblast apoptosis by targeting SIRT1. Thus, the miR-138-5p/SIRT1 pathway should be considered for preventative treatment of disuse osteoporosis.