Prebiotic potential of pectin and pectic oligosaccharides to promote anti-inflammatory commensal bacteria in the human colon. Issue 11 (3rd October 2017)

• Record Type: Journal Article
• Authors: Chung, Wing Sun Faith; Meijerink, Marjolein; Zeuner, Birgitte; Holck, Jesper; Louis, Petra; Meyer, Anne S.; Wells, Jerry M.; Flint, Harry J.; Duncan, Sylvia H.
• Is Part Of: FEMS microbiology ecology. Volume 93:Issue 11(2017:Nov.)
• Publication Date: 2017-10-03
• Subjects: Firmicutes -- Eubacterium eligens -- Faecalibacterium prausnitzii -- Bacteroides thetaiotaomicron -- glycosyl hydrolase -- pectate lyase; Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1093/femsec/fix127
• Languages: English
• ISSNs: 0168-6496

Abstract

Abstract: Dietary plant cell wall carbohydrates are important in modulating the composition and metabolism of the complex gut microbiota, which can impact on health. Pectin is a major component of plant cell walls. Based on studies in model systems and available bacterial isolates and genomes, the capacity to utilise pectins for growth is widespread among colonic Bacteroidetes but relatively uncommon among Firmicutes. One Firmicutes species promoted by pectin is Eubacterium eligens. E ubacterium eligens DSM3376 utilises apple pectin and encodes a broad repertoire of pectinolytic enzymes, including a highly abundant pectate lyase of around 200 kDa that is expressed constitutively. We confirmed that certain Faecalibacterium prausnitzii strains possess some ability to utilise apple pectin and report here that F. prausnitzii strains in common with E. eligens can utilise the galacturonide oligosaccharides DP4 and DP5 derived from sugar beet pectin. F aecalibacterium prausnitzii strains have been shown previously to exert anti-inflammatory effects on host cells, but we show here for the first time that E. eligens strongly promotes the production of the anti-inflammatory cytokine IL-10 in in vitro cell-based assays. These findings suggest the potential to explore further the prebiotic potential of pectin and its derivatives to re-balance the microbiota towards an anti-inflammatory profile. Abstract : Eubacterium eligens is a key member of the healthy human colonic microbiota which grows well on pectin and promotes potent anti-inflammatory activity.