Intraspecies polymorphisms of Kluyveromyces marxianus strains from Yaghnob valley. Issue 6 (1st February 2018)

• Record Type: Journal Article
• Authors: Perpetuini, Giorgia; Tittarelli, Fabrizia; Mattarelli, Paola; Modesto, Monica; Cilli, Elisabetta; Suzzi, Giovanna; Tofalo, Rosanna
• Is Part Of: FEMS microbiology letters. Volume 365:Issue 6(2018:Mar.)
• Publication Date: 2018-02-01
• Subjects: Kluyveromyces marxianus -- Biolog -- fermented goat milk -- PFGE -- adhesion properties -- MAT; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fny028
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strains differed in terms of number and size of chromosome bands. The number of bands ranged from 5 to 7, suggesting a probable genome size from 1.4 to 2.6 Mb. Strains showed a certain level of cell surface hydrophobicity ranging from 32% to 77.7%. Strains were also tested for their ability to form a biofilm on polystyrene plates: planktonic cells ranged from 6.3 cfu/mL to 7.95 cfu/mL, while sessile from 7.11 cfu/mL to 8.6 cfu/mL. The strains able to adhere to polystyrene plates were also able to form a mature MAT. Biolog analysis revealed that almost all strains were able to use putrescine, malic acid, α-D lactose, phenylethylamine, β-methyl D-gucoside and xylose; 5 strains were able to grow on cellobiose and 3 were able to catabolise α-ketobutyric. The obtained data highlighted a number of interesting features underlying the peculiar capacities of these strains for industrial applications. Abstract : Kluyveromyces marxianus strains isolated from the Yaghnob valley showed high degree of intraspecies polymorphism in terms of chromosome numbers, adhesion properties and metabolic potential that could be exploited for industrial applications.