Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase. Issue 49 (4th June 2018)

• Record Type: Journal Article
• Authors: Barnaba, Carlo; Ravula, Thirupathi; Medina-Meza, Ilce G.; Im, Sang-Choul; Anantharamaiah, G. M.; Waskell, Lucy; Ramamoorthy, Ayyalusamy
• Is Part Of: Chemical communications. Volume 54:Issue 49(2018)
• Page Start: 6336
• Page End: 6339
• Publication Date: 2018-06-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc02003e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Lipids are critical for the function of mammalian cytochrome-P450s. Abstract : Lipids are critical for the function of membrane proteins. NADPH–cytochrome-P450-reductase, the sole electron transferase for microsomal oxygenases, possesses a conformational dynamics entwined with its topology. Here, we use peptide-nanodiscs to unveil cytochrome-P450-reductase's lipid boundaries, demonstrating a protein-driven enrichment of ethanolamine lipids (by 25%) which ameliorates by 3-fold CPR's electron-transfer ability.