A detailed redescription of the mesoderm/neural crest cell boundary in the murine orbitotemporal region integrates the mammalian cranium into a pan‐amniote cranial configuration. Issue 1 (31st July 2022)

• Record Type: Journal Article
• Authors: Kuroda, Shunya; Adachi, Noritaka; Kuratani, Shigeru
• Other Names: Albertson Craig guestEditor.; Evans Kory guestEditor.
• Is Part Of: Evolution & development. Volume 25:Issue 1(2023)
• Page Start: 32
• Page End: 53
• Publication Date: 2022-07-31
• Subjects: mammal -- morphology -- neurocranium; Evolution (Biology) -- Periodicals; Developmental biology -- Periodicals; 576.82
• DOI: 10.1111/ede.12411
• Languages: English
• ISSNs: 1520-541X

Abstract

Abstract: The morphology of the mammalian chondrocranium appears to differ significantly from those of other amniotes, since the former possesses uniquely developed brain and cranial sensory organs. In particular, a question has long remained unanswered as to the developmental and evolutionary origins of a cartilaginous nodule called the ala hypochiasmatica. In this study, we investigated the embryonic origin of skeletal elements in the murine orbitotemporal region by combining genetic cell lineage analysis with detailed morphological observation. Our results showed that the mesodermal embryonic environment including the ala hypochiasmatica, which appeared as an isolated mesodermal distribution in the neural crest‐derived prechordal region, is formed as a part of the mesoderm that continued from the chordal region during early chondrocranial development. The mesoderm/neural crest cell boundary in the head mesenchyme is modified through development, resulting in the secondary mesodermal expansion to invade into the prechordal region. We thus revealed that the ala hypochiasmatica develops as the frontier of the mesodermal sheet stretched along the cephalic flexure. These results suggest that the mammalian ala hypochiasmatica has evolved from a part of the mesodermal primary cranial wall in ancestral amniotes. In addition, the endoskeletal elements in the orbitotemporal region, such as the orbital cartilage, suprapterygoid articulation of the palatoquadrate, and trabecula, some of which were once believed to represent primitive traits of amniotes and to be lost in the mammalian lineage, have been confirmed to exist in the mammalian cranium. Consequently, the mammalian chondrocranium can now be explained in relation to the pan‐amniote cranial configuration. Abstract : In the murine chondrocranium, the ala hypochiasmatica appears to remain as isolated nodules in the neural crest‐derived prechordal cranium (colored in light blue). In fact, however, the ala hypochiasmatica is not independent from the mesodermal region of the chordal cranium (colored in pink); it is a part of the mesodermal population contiguous with the chordal cranium via a mesodermal primary cranial wall that appears as a mesenchymal sheet (transparent pink region).