Comparison of the interleukin 27 expression for the acute and chronic phase of mononucleosis in children. (May 2021)

• Record Type: Journal Article
• Authors: Zelazowska-Rutkowska, Beata; Niewinska, Gabriela; Toczylowski, Kacper; Cylwik, Bogdan
• Is Part Of: Cytokine. Volume 141(2021)
• Publication Date: 2021-05
• Subjects: Interleukin 27 -- Mononucleosis -- Acute and chronic phase; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2020.155395
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: Serum level of IL-27 was found to be significantly higher in children with mononucleosis in comparison with healthy subjects. Serum concentration of IL- 27 in children with IM is increased, thus confirming the on-going inflammatory process. IL-27 can be a useful indicator to differentiate between the acute and chronic phase of the disease. The serum IL-27 positively correlated with ALT, AST and LDH activities in the whole study group. Abstract: Background: The objective of the current study was to assess the serum level of interleukin 27 in children with mononucleosis and to compare the expression of this cytokine in the acute and chronic phase of the infection. Methods: The level of IL-27 was determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (Diaclone SAS, Besancon, France). Other laboratory findings were determined using routine laboratory methods. Results: Serum level of IL-27 was found to be significantly higher in children with mononucleosis in comparison with healthy subjects (almost a 4-fold increase, 15.7 vs. 4.2 pg/mL, p < 0.001). It was also significantly higher in the acute phase compared to the chronic stage of the disease (more than a two-fold increase, 20.7 vs. 9.64 pg/mL, p < 0.001). This cytokine positively correlated with ALT, AST, LDH activity and WBC count (R = 0.498, p < 0.001; R = 0.586, p < 0.001; R = 0.170, p < 0.05, R = 0.329, p < 0.05, respectively) in the whole study, and only with AST activity in the chronic phase subgroup (R = 0.684, p < 0.05). Conclusion: In conclusion, this study shows that serum concentration of interleukin 27 in children with mononucleosis is increased, thus confirming the on-going inflammatory process. We also suggest that IL-27 can be a useful indicator to differentiate between the acute and chronic phase of the disease.