Effects of ND vaccination combined LPS on growth performance, antioxidant performance and lipid metabolism of broiler. (March 2021)

• Record Type: Journal Article
• Authors: Li, Xiaowen; Liu, Shuiping; Wang, Ji; Yi, Jine; Yuan, Zhihang; Wu, Jing; Wen, Lixin; Li, Rongfang
• Is Part Of: Research in veterinary science. Volume 135(2021)
• Page Start: 317
• Page End: 323
• Publication Date: 2021-03
• Subjects: Immune stress -- Lipopolysaccharide -- Growth performance -- Antioxidant ability -- Lipid metabolism -- Broiler; ACC Acetyl-CoA carboxylase -- ADFI Average daily feed intake -- ADG Average daily gain -- CPT-1 Carnitine palmitoyltransferase-1 -- FCR Feed conversion ratio -- GSH-Px Glutathione peroxidase -- HDL-C High-density lipoprotein cholesterol -- HMGR 3-hydroxy-3-methylglutaryl coenzyme-A reductase -- LDL-C Low-density lipoprotein cholesterol -- LPS Lipopolysaccharide -- MDA Malondialdehyde -- ND Newcastle Disease -- PPAR-α Peroxisome proliferator-activated receptor -- TC Total cholesterol -- TG Triglyceride -- T-SOD Total superoxide dismutase; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2020.10.007
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Newcastle Disease Virus (NDV) is the important pathogen of Newcastle Disease (ND) attacking chicken, turkey and other birds. Therefore, the purpose of this study was to assess the effects of immune stress induced by ND vaccination and lipopolysaccharide (LPS) on growth performance, antioxidant ability, and lipid metabolism of broilers. In total, 128 one-day-old broilers were randomly assigned to the following four groups and were treated as indicated: normal control (NC); vaccinated with live LaSota ND vaccine (CV); administered ND vaccine and 0.25 mg/kg body weight (BW) LPS (L-LPS); and administered ND vaccine and 0.5 mg/kg BW LPS (H-LPS). The results demonstrated that broiler feed conversion ratio (FCR) was increased in the groups CV, L-LPS and H-LPS from d 0 to 42 days compared with the group NC. The antioxidant function of broilers was decreased as indicated by the malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels in the serum of the treated groups. ND vaccination combined LPS increased the concentration of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), but decreased the concentration of high-density lipoprotein cholesterol (HDLC) compared with the group NC. The reverse transcription (RT)-PCR results revealed that the mRNA expression of acetyl-CoA carboxylase gene (ACC) and 3-hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR) in the liver were downregulated, whereas the mRNA expression of carnitine palmitoyltransferase-1 (CPT-1) and peroxisome proliferator-activated receptor (PPAR)-α were upregulated compared with the group NC. These results suggest that ND vaccination combined LPS reduced broiler growth performance and antioxidant ability, whereas it activated AMPK-mediated lipid metabolism. Highlights: LPS peritoneal injection combined with NDV immunization could be a feasible model for immune stress. ND vaccination combined LPS reduced the total weight ratio, FCR, and antioxidant function of broilers. ND vaccination combined LPS on cholesterol was completely regulated by the AMPK lipid metabolism pathway.