Comparison of Freshly Isolated Adipose Tissue-derived Stromal Vascular Fraction and Bone Marrow Cells in a Posterolateral Lumbar Spinal Fusion Model. Issue 10 (15th May 2021)

• Record Type: Journal Article
• Authors: Perdomo-Pantoja, Alexander; Holmes, Christina; Cottrill, Ethan; Rindone, Alexandra N.; Ishida, Wataru; Taylor, Maritza; Tomberlin, Colson; Lo, Sheng-fu L.; Grayson, Warren L.; Witham, Timothy F.
• Is Part Of: Spine. Volume 46:Issue 10(2021)
• Page Start: 631
• Page End: 637
• Publication Date: 2021-05-15
• Subjects: adipose-derived stromal vascular fraction -- bone marrow cells -- mesenchymal stromal cell -- rat posterolateral fusion model -- spinal fusion; Spine -- Abnormalities -- Periodicals; Spine -- Diseases -- Periodicals; Spine -- Surgery -- Periodicals; 616.73005
• DOI: 10.1097/BRS.0000000000003709
• Languages: English
• ISSNs: 0362-2436

Abstract

Abstract : Supplemental Digital Content is available in the text Abstract : Study Design: Rat posterolateral lumbar fusion model. Objective: The aim of this study was to compare the efficacy of freshly isolated adipose tissue-derived stromal vascular fraction (A-SVF) and bone marrow cells (BMCs) cells in achieving spinal fusion in a rat model. Summary of Background Data: Adipose tissue-derived stromal cells (ASCs) offer advantages as a clinical cell source compared to bone marrow-derived stromal cells (BMSCs), including larger available tissue volumes and reduced donor site morbidity. While pre-clinical studies have shown that ex vivo expanded ASCs can be successfully used in spinal fusion, the use of A-SVF cells better allows for clinical translation. Methods: A-SVF cells were isolated from the inguinal fat pads, whereas BMCs were isolated from the long bones of syngeneic 6- to 8-week-old Lewis rats and combined with Vitoss (Stryker) bone graft substitute for subsequent transplantation. Posterolateral spinal fusion surgery at L4-L5 was performed on 36 female Lewis rats divided into three experimental groups: Vitoss bone graft substitute only (VO group); Vitoss + 2.5 × 10 6 A-SVF cells/side; and, Vitoss + 2.5 × 10 6 BMCs/side. Fusion was assessed 8 weeks post-surgery via manual palpation, micro-computed tomography (μCT) imaging, and histology. Results: μCT imaging analyses revealed that fusion volumes and μCT fusion scores in the A-SVF group were significantly higher than in the VO group; however, they were not significantly different between the A-SVF group and the BMC group. The average manual palpation score was highest in the A-SVF group compared with the BMC and VO groups. Fusion masses arising from cell-seeded implants yielded better bone quality than nonseeded bone graft substitute. Conclusion: In a rat model, A-SVF cells yielded a comparable fusion mass volume and radiographic rate of fusion to BMCs when combined with a clinical-grade bone graft substitute. These results suggest the feasibility of using freshly isolated A-SVF cells in spinal fusion procedures. Level of Evidence: N/A