Potential prebiotic substrates modulate composition, metabolism, virulence and inflammatory potential of an in vitro multi-species oral biofilm. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Verspecht, Tim; Van Holm, Wannes; Boon, Nico; Bernaerts, Kristel; Daep, Carlo A; Masters, James G; Zayed, Naiera; Quirynen, Marc; Teughels, Wim
• Is Part Of: Journal of oral microbiology. Volume 13:Issue 1(2021)
• Publication Date: 2021-01-01
• Subjects: Prebiotics -- prebiotic substrates -- oral health -- multi-species oral biofilm -- oral biofilm composition -- oral biofilm metabolism -- oral biofilm virulence -- oral biofilm inflammatory potential; Mouth -- Microbiology -- Periodicals; Medical microbiology -- Periodicals; Mouth Diseases -- microbiology; Mouth -- microbiology; Medical microbiology; Mouth -- Microbiology; Oral Pathology; Microbiology; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.31
• DOI: 10.1080/20002297.2021.1910462
• Languages: English
• ISSNs: 2000-2297

Abstract

ABSTRACT: Background : Modulation of the commensal oral microbiota constitutes a promising preventive/therapeutic approach in oral healthcare. The use of prebiotics for maintaining/restoring the health-associated homeostasis of the oral microbiota has become an important research topic. Aims : This study hypothesised that in vitro 14-species oral biofilms can be modulated by (in)direct stimulation of beneficial/commensal bacteria with new potential prebiotic substrates tested at 1 M and 1%(w/v), resulting in more host-compatible biofilms with fewer pathogens, decreased virulence and less inflammatory potential. Methods : Established biofilms were repeatedly rinsed with N-acetyl-D-glucosamine, α-D-lactose, D-(+)-trehalose or D-(+)-raffinose at 1 M or 1%(w/v) . Biofilm composition, metabolic profile, virulence and inflammatory potential were eventually determined. Results : Repeated rinsing caused a shift towards a more health-associated microbiological composition, an altered metabolic profile, often downregulated virulence gene expression and decreased the inflammatory potential on oral keratinocytes. At 1 M, the substrates had pronounced effects on all biofilm aspects, whereas at 1%(w/v) they had a pronounced effect on virulence gene expression and a limited effect on inflammatory potential. Conclusion : Overall, this study identified four new potential prebiotic substrates that exhibit different modulatory effects at two different concentrations that cause in vitro multi-species oral biofilms to become more host-compatible.