Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation. Issue 1 (1st January 2021)

• Record Type: Journal Article
• Authors: Mamana, Ana Carolina; Stincarelli, Maria; Sarmento, Dmitry José De Santana; Mendes Batista, Alexandre; Tozetto-Mendoza, Tânia Regina; Gallottini, Marina; De Abreu Pestana, José Osmar Medina; Braz-Silva, Paulo Henrique; Giannecchini, Simone
• Is Part Of: Journal of oral microbiology. Volume 13:Issue 1(2021)
• Publication Date: 2021-01-01
• Subjects: Polyomavirus -- polyomavirus microRNA-5p -- saliva -- renal transplantation -- polyomavirus-associated diseases; Mouth -- Microbiology -- Periodicals; Medical microbiology -- Periodicals; Mouth Diseases -- microbiology; Mouth -- microbiology; Medical microbiology; Mouth -- Microbiology; Oral Pathology; Microbiology; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.31
• DOI: 10.1080/20002297.2021.1898838
• Languages: English
• ISSNs: 2000-2297

Abstract

ABSTRACT: Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.