A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163. Issue 2 (February 2014)

• Record Type: Journal Article
• Authors: Mohedano, María de la Luz; Russo, Pasquale; de los Ríos, Vivian; Capozzi, Vittorio; Fernández de Palencia, Pilar; Spano, Giuseppe; López, Paloma
• Is Part Of: Open biology. Volume 4:Issue 2(2014)
• Publication Date: 2014-02
• Subjects: Oenococcus oeni -- proteome -- two-dimensional electrophoresis; Biology -- Periodicals; 570
• DOI: 10.1098/rsob.130154
• Languages: English
• ISSNs: 2046-2441

Abstract

Abstract : Oenococcus oeni is the main lactic acid bacterium that carries out the malolactic fermentation in virtually all red wines and in some white and sparkling wines. Oenococcus oeni possesses an array of metabolic activities that can modify the taste and aromatic properties of wine. There is, therefore, industrial interest in the proteins involved in these metabolic pathways and related transport systems of this bacterium. In this work, we report the characterization of the O. oeni ATCC BAA-1163 proteome. Total and membrane protein preparations from O. oeni were standardized and analysed by two-dimensional gel electrophoresis. Using tandem mass spectrometry, we identified 224 different spots corresponding to 152 unique proteins, which have been classified by their putative function and subjected to bioinformatics analysis.