Structural characterization of alkali-soluble polysaccharides from Panax ginseng C. A. Meyer. Issue 3 (14th March 2018)

• Record Type: Journal Article
• Authors: Ji, Li; Jie, Zhenjing; Ying, Xin; Yue, Qi; Zhou, Yifa; Sun, Lin
• Is Part Of: Royal Society open science. Volume 5:Issue 3(2018)
• Publication Date: 2018-03-14
• Subjects: ginseng -- polysaccharides -- pectin -- hemicellulose -- structural analysis; Science -- Periodicals; 500
• DOI: 10.1098/rsos.171644
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : Panax ginseng C. A. Meyer (ginseng) has been widely used as a herb and functional food in the world. Polysaccharides are the main active components of ginseng. In this paper, the polysaccharides were sequentially extracted by 50 mM Na2 CO3, 1 M KOH and 4 M KOH from ginseng roots treated sequentially with hot water, α-amylase and ethylenediaminetetraacetic acid extraction. Na2 CO3 -soluble ginseng polysaccharide (NGP) was fractionated into one neutral and three acidic fractions by anion exchange and gel permeation chromatography. Fourier transform infrared, NMR and methylation analysis indicated acidic fractions in NGP were highly branched rhamnogalacturonan-I domains, with → 4)-α-Gal p A-(1 → 2)-α-Rha p -(1 → disaccharide repeating units as backbone and β-1, 4-galactan, α-1, 5/1, 3, 5-arabinan and type II arabinogalactan as side chains. 1-KGP (1 M KOH-soluble ginseng polysaccharide) and 4-KGP (4 M KOH-soluble ginseng polysaccharide) were mainly composed of hemicellulose besides starch-like polysaccharides and minor pectin. Antibody detection, enzymic hydrolysis, high performance anion exchange chromatography and methylation analysis demonstrated xylan was the major component in 1-KGP, while xyloglucan was predominant in 4-KGP. Comparing the polysaccharides obtained by different solvent extractions, we have a comprehensive understanding about total ginseng polysaccharides.