Micro/nano-hierarchical structured TiO2 coating on titanium by micro-arc oxidation enhances osteoblast adhesion and differentiation. Issue 4 (24th April 2019)

• Record Type: Journal Article
• Authors: Pan, Xumeng; Li, Yada; Abdullah, Adil O.; Wang, Weiqiang; Qi, Min; Liu, Yi
• Is Part Of: Royal Society open science. Volume 6:Issue 4(2019)
• Publication Date: 2019-04-24
• Subjects: micro-arc oxidation -- titanium -- MG63 -- osteogenic differentiation -- dental implants; Science -- Periodicals; 500
• DOI: 10.1098/rsos.182031
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : Nano-structured and micro/nano-hierarchical structured TiO2 coatings were produced on polished titanium by the micro-arc oxidation (MAO) technique. This study was conducted to screen a suitable structured TiO2 coating for osteoblast adhesion and differentiation in dental implants. The formulation was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and wettability testing. Adhesion, proliferation and osteogenic differentiation of MG63 cells were analysed by SEM, Cell Counting Kit-8 (CCK-8) and quantitative real-time PCR. The micro/nano-hierarchical structured TiO2 coating with both slots and pores showed the best morphology and wettability. XRD analysis revealed that rutile predominated along with a minor amount of anatase in both TiO2 coatings. Adhesion and extension of MG63 cells on the micro/nano-hierarchical structured TiO2 coating were the most favourable. MG63 cells showed higher growth rates on the micro/nano-hierarchical structured TiO2 coating at 1 and 3 days. Osteogenic-related gene expression was markedly increased in the micro/nano-hierarchical structured TiO2 coating group compared with the polished titanium group at 7, 14 and 21 days. These results revealed the micro/nano-hierarchical structured TiO2 coating as a promising surface modification and suitable biomaterial for use with dental implants.