Development of the HPLC–ELSD method for the determination of phytochelatins and glutathione in Perilla frutescens under cadmium stress conditions. Issue 5 (9th May 2018)

• Record Type: Journal Article
• Authors: Zheng, Xinyu; Chen, Shen; Zheng, Meiqin; Peng, Jun; He, Xiaosan; Han, Yongming; Zhu, Jingjing; Xiao, Qingtie; Lv, Rixin; Lin, Ruiyu
• Is Part Of: Royal Society open science. Volume 5:Issue 5(2018)
• Publication Date: 2018-05-09
• Subjects: HPLC–ELSD -- glutathione -- phytochelatins -- Perilla frutescens; Science -- Periodicals; 500
• DOI: 10.1098/rsos.171659
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : A rapid, accurate and simple method was developed for the simultaneous determination of glutathione (GSH) and phytochelatins (PCs) by high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector. GSH, phytochelatin 2 (PC2 ), PC3, PC4, PC5 and PC6 can be separated with baseline separation within 9 min using a Venusil AA column (250 mm × 4.6 mm i.d., 5 µm particle sizes). Acetonitrile and water containing 0.1% trifluoroacetic acid (0.1%) were employed as the mobile phase for the gradient elution. The drift tube temperature and flow rate of the carrier gas (N2 ) were 50°C and 1.5 l min −1, respectively. Under optimum conditions, good linear regression equations of six analytes were obtained with the detection limits ranging from 0.2 to 0.5 µg ml −1 . The proposed method has been applied successfully for the quantification of GSH and PCs in Perilla frutescens (a cadmium hyperaccumulator) under cadmium stress. The recoveries were between 82.9% and 115.3%.