Comparative chemical analysis of volatile compounds of Echinops ilicifolius using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil. Issue 2 (14th February 2018)

• Record Type: Journal Article
• Authors: Mohebat, Razieh; Bidoki, Mina Zare
• Is Part Of: Royal Society open science. Volume 5:Issue 2(2018)
• Publication Date: 2018-02-14
• Subjects: Echinops Ilicifolius -- compositae -- hydrodistillation -- HS-SPME -- antibacterial activity -- essential oil; Science -- Periodicals; 500
• DOI: 10.1098/rsos.171424
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : The volatile compounds from various parts of Echinops ilicifolius (Compositae) such as flowers, leaves and roots obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) methods were subsequently analysed by GC and GC/MS and compared. Thirty-seven and 20 components of the flower of E. ilicifolius were characterized, representing 99.7% and 100.0% of the total compositions using the HD and HS-SPME methods, respectively. The major constituents of the hydrodistilled oil were identified as linalool (58.6%), geraniol (17.4%), n -dodecane (10.9%) and nerol (5.4%), whereas HS-SPME extract was rich in n -octane (34.0%), n -decane (25.1%), p -cymene (11.1%), γ -terpinene (5.3%) and 1, 8-cineole (5.1%). n -Hexadecanoic acid (32.3%), linalool (16.4%) and geraniol (8.3%) were the main components among 43 constituents identified in hydrodistilled extract of the leaf, representing 99.5% of the total components detected, whereas 16 compounds representing 99.9% of the HS-SPME method were identified, among which n -decane (32.6%), p -cymene (14.0%), n -octane (10.3%), limonene (9.2%), γ -terpinene (9.1%), 1, 8-cineole (7.9%) and α -pinene (5.9%) were the major ones. Among 23 components comprising 91.2% of the total hydrodistilled oil detected, n -decane (23.1%), n -dodecane (14.5%), silphiperfol-4, 7(14)-diene (11.1%), selin-11-en-4-a-ol (9.5%), n -hexadecanoic acid (7.8%) and n -tetradecane (5.3%) were the main constituents in the root of E. ilicifolius, whereas 1, 8-cineole (29.0%), n -decane (12.6%), n -octane (12.6%), camphor (12.0%), p -cymene (9.6%) and γ -terpinene (5.9%) were the main components among the 20 constituents characterized in HS-SPME extract, representing 99.9% of the total components detected. The antibacterial activity of the flower, leaf and root oils of E. ilicifolius against six Gram-positive and Gram-negative bacteria was determined using the MIC method. The growth inhibitory zone (mm) was also measured.