Minimally Invasive Microbiopsies as an Improved Sampling Method for the Diagnosis of Cutaneous Leishmaniasis. (17th August 2020)

• Record Type: Journal Article
• Authors: Churiso, Gemechu; van Henten, Saskia; Cnops, Lieselotte; Pollmann, Julia; Melkamu, Roma; Lemma, Mulualem; Kiflie, Amare; Fikre, Helina; van Griensven, Johan; Adriaensen, Wim
• Is Part Of: Open forum infectious diseases. Volume 7:Number 9(2020)
• Publication Date: 2020-08-17
• Subjects: cutaneous leishmaniasis -- diagnosis -- Leishmania aethiopica -- sample collection; Communicable diseases -- Periodicals; Medical microbiology -- Periodicals; Infection -- Periodicals; 616.9
• DOI: 10.1093/ofid/ofaa364
• Languages: English
• ISSNs: 2328-8957

Abstract

Abstract: Current sampling methods to diagnose cutaneous leishmaniasis are invasive and painful. An alternative and minimally invasive microbiopsy device was evaluated in a diverse range of cutaneous leishmaniasis lesions in Ethiopia. Using polymerase chain reaction–based diagnosis, the microbiopsy outperformed the routine skin slit sample by detecting more patients while pain scores were significantly lower. Abstract : Current sampling methods to diagnose cutaneous leishmaniasis are invasive and painful. In diverse lesion types and using PCR-based diagnosis, a novel and minimally-invasive microbiopsy outperformed the routine skin slit sample by detecting more patients while pain scores were significantly lower.