Mucosal Invariant T cells are Diminished in Very Early-Onset Inflammatory Bowel Disease. Issue 4 (October 2021)

• Record Type: Journal Article
• Authors: Dou, Ying; Maurer, Kelly; Conrad, Maire; Patel, Trusha; Shraim, Rawan; Sullivan, Kathleen E.; Kelsen, Judith
• Is Part Of: Journal of pediatric gastroenterology and nutrition. Volume 73:Issue 4(2021)
• Publication Date: 2021-10
• Subjects: innate lymphoid cell -- innate lymphoid cells -- mucosal-associated invariant T -- microbiota -- very early-onset inflammatory bowel disease; Children -- Nutrition -- Periodicals; Pediatric gastroenterology -- Periodicals; Infants -- Nutrition -- Periodicals; Nutrition disorders in children -- Periodicals; Child Nutrition -- Periodicals; Digestive System -- growth & development -- Periodicals; Gastrointestinal Diseases -- Periodicals; Infant Nutrition -- Periodicals; Nutrition Disorders -- Periodicals; Child; 618.923
• DOI: 10.1097/MPG.0000000000003189
• Languages: English
• ISSNs: 0277-2116

Abstract

ABSTRACT: Objectives: Very early-onset inflammatory bowel disease (VEO-IBD) arises in children less than 6 years old, a critical time for immunologic development and maturation of the intestinal microbiome. Non-conventional lymphocytes, defined here as mucosal-associated invariant T cells and innate lymphocytes, require microbial products for either development or expansion, aspects that could be altered in very early-onset inflammatory bowel disease. Our objective was to define conventional leukocyte and non-conventional lymphocyte populations in controls and patients using multiparameter flow cytometry to test the hypothesis that their frequencies would be altered in a chronic inflammatory state associated with significant dysbiosis. Methods: Multiparameter flow cytometry was used in a control cohort of 105 subjects to define age-effects, not previously comprehensively examined for these cell types in humans. Differences were defined between 263 unique age-matched patients with VEO-IBD and 105 controls using Student t -test. Subjects were divided into two age groups at the time of sampling to control for age-related changes in immune composition. Results: Intermediate monocytes were consistently decreased in patients with VEO-IBD compared to controls. Mucosal-associated invariant T cells were significantly lower in patients with long-standing disease. Levels were less than half of those seen in the age-matched control cohort. The innate lymphoid cells type 2 population was expanded in the youngest patients. Conclusion: Mucosal-associated invariant T cells are diminished years after presentation with inflammatory bowel disease. This durable effect of early life intestinal inflammation may have long-term consequences. Diminished mucosal-associated invariant T cells could impact host defense of intestinal infections. Abstract : Supplemental Digital Content is available in the text