Controlled fabrication of drug‐loaded protein nanoparticles via flash nanoprecipitation. Issue 2 (6th November 2022)

• Record Type: Journal Article
• Authors: Zhao, Hongyang; Wang, Mingwei; Wang, Xinming; Liu, Jinbo; Xing, Mengyuan; Huang, Haiyan; Cohen Stuart, Martien A.; Wang, Junyou
• Is Part Of: AIChE journal. Volume 69:Issue 2(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-11-06
• Subjects: controlled fabrication -- drug delivery -- flash nanoprecipitation -- protein nanoparticles -- regulated properties; Chemical engineering -- Periodicals; Génie chimique -- Périodiques; 660.28
• DOI: 10.1002/aic.17941
• Languages: English
• ISSNs: 0001-1541

Abstract

Abstract: Protein nanoparticles (NPs) are perspective vehicles for delivery of therapeutic agents. However, whether they will ever reach the stage of widespread application relies crucially on the development of reliable fabrication strategies and techniques. Herein, we report a continuous precipitation approach, namely flash nanoprecipitation (FNP) for efficient construction of drug‐loaded protein NPs with regulated size and properties. Our design relies on rapid mixing of solvent streams containing drug and protein with anti‐solvent streams which triggers co‐precipitation of the solutes and creates NPs by controlled nucleation and growth. The established process is generally capable for controlled formulation of diverse drug‐loaded protein NPs. Our results validate the distinctive advantages of FNP over conventional precipitation approaches, including fast process, continuous production and excellent control of product size and properties. FNP is therefore likely to be the method of choice for preparing nanosized dispersions of hydrophobic drugs or food additives on an industrial scale.