Intestinal enteroendocrine cells rely on ryanodine and IP3 calcium store receptors for mechanotransduction. (13th December 2022)

• Record Type: Journal Article
• Authors: Knutson, Kaitlyn R.; Whiteman, Sara T.; Alcaino, Constanza; Mercado‐Perez, Arnaldo; Finholm, Isabelle; Serlin, Hannah K.; Bellampalli, Shreya S.; Linden, David R.; Farrugia, Gianrico; Beyder, Arthur
• Is Part Of: Journal of physiology. Volume 601:Number 2(2023)
• Page Start: 287
• Page End: 305
• Publication Date: 2022-12-13
• Subjects: enteroendocrine cells -- intracellular stores -- mechanotransduction; Physiology -- Periodicals; 612.005
• DOI: 10.1113/JP283383
• Languages: English
• ISSNs: 0022-3751

Abstract

Abstract : Abstract: Enteroendocrine cells (EECs) are specialized sensors of luminal forces and chemicals in the gastrointestinal (GI) epithelium that respond to stimulation with a release of signalling molecules such as serotonin (5‐HT). For mechanosensitive EECs, force activates Piezo2 channels, which generate a very rapidly activating and inactivating (∼10 ms) cationic (Na +, K +, Ca 2+ ) receptor current. Piezo2 receptor currents lead to a large and persistent increase in intracellular calcium (Ca 2+ ) that lasts many seconds to sometimes minutes, suggesting signal amplification. However, intracellular calcium dynamics in EEC mechanotransduction remain poorly understood. The aim of this study was to determine the role of Ca 2+ stores in EEC mechanotransduction. Mechanical stimulation of a human EEC cell model (QGP‐1) resulted in a rapid increase in cytoplasmic Ca 2+ and a slower decrease in ER stores Ca 2+, suggesting the involvement of intracellular Ca 2+ stores. Comparing murine primary colonic EECs with colonocytes showed expression of intercellular Ca 2+ store receptors, a similar expression of IP3 receptors, but a >30‐fold enriched expression of Ryr3 in EECs. In mechanically stimulated primary EECs, Ca 2+ responses decreased dramatically by emptying stores and pharmacologically blocking IP3 and RyR1/3 receptors. RyR3 genetic knockdown by siRNA led to a significant decrease in mechanosensitive Ca 2+ responses and 5‐HT release. In tissue, pressure‐induced increase in the Ussing short circuit current was significantly decreased by ryanodine receptor blockade. Our data show that mechanosensitive EECs use intracellular Ca 2+ stores to amplify mechanically induced Ca 2+ entry, with RyR3 receptors selectively expressed in EECs and involved in Ca 2+ signalling, 5‐HT release and epithelial secretion. Key points: A population of enteroendocrine cells (EECs) are specialized mechanosensors of the gastrointestinal (GI) epithelium that respond to mechanical stimulation with the release of important signalling molecules such as serotonin. Mechanical activation of these EECs leads to an increase in intracellular calcium (Ca 2+ ) with a longer duration than the stimulus, suggesting intracellular Ca 2+ signal amplification. In this study, we profiled the expression of intracellular Ca 2+ store receptors and found an enriched expression of the intracellular Ca 2+ receptor Ryr3, which contributed to the mechanically evoked increases in intracellular calcium, 5‐HT release and epithelial secretion. Our data suggest that mechanosensitive EECs rely on intracellular Ca 2+ stores and are selective in their use of Ryr3 for amplification of intracellular Ca 2+ . This work advances our understanding of EEC mechanotransduction and may provide novel diagnostic and therapeutic targets for GI motility disorders. Abstract : Abstract Figure Legend Mechanosensitive enteroendocrine cells (EECs) found in the intestinal epithelium express the mechanosensitive ion channel Piezo2. Force activates Piezo2 channels, which upon activation generate a very rapidly activating and inactivating (∼10 ms) cationic receptor current. Yet, rapid Piezo2 receptor currents lead to a large and persistent increase in intracellular calcium (Ca 2+ ) that lasts many seconds to sometimes minutes, suggesting signal amplification. This study has found that the signal amplification involves calcium‐induced calcium release (CICR) mechanisms activated through intracellular stores receptors, RyR3 and IP3Rs. Activation of these receptors leads to a large and sustained increase in Ca 2+ that results in the release of serotonin (5‐HT) that plays an important role in many local and systemic processes, such as pressure‐induced secretion.