Structural characterization of collagen using polarization-resolved second harmonic generation (SHG) microscopy. Issue 1 (March 2021)

• Record Type: Journal Article
• Authors: Mazumder, N; Sindhoora, K M; Kao, F-J
• Is Part Of: Journal of physics. Volume 1859:Issue 1(2021)
• Publication Date: 2021-03
• Subjects: Physics -- Congresses; 530.5
• DOI: 10.1088/1742-6596/1859/1/012034
• Languages: English
• ISSNs: 1742-6588

Abstract

Abstract: Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.