A new isolate of Streptomyces lateritius (Z1‐26) with antibacterial activity against fish pathogens and immune enhancement effects on crucian carp (Carassius auratus). Issue 2 (20th October 2022)

• Record Type: Journal Article
• Authors: Yang, Yahui; Jin, Duo; Long, Wensu; Lai, Ximiao; Sun, Yunjun; Zhai, Feng; Wang, Pan; Zhou, Xixun; Hu, Yibo; Xia, Liqiu; Yi, Ganfeng
• Is Part Of: Journal of fish diseases. Volume 46:Issue 2(2023)
• Page Start: 99
• Page End: 112
• Publication Date: 2022-10-20
• Subjects: antibacterial activity -- Carassius auratus -- genome sequencing -- growth -- immune response; Fishes -- Diseases -- Periodicals; 639.3
• DOI: 10.1111/jfd.13723
• Languages: English
• ISSNs: 0140-7775

Abstract

Abstract: The Streptomyces lateritius Z1‐26 was isolated from soil samples which showed broad‐spectrum antibacterial activity against a broad range of fish pathogens. The In Vivo Imaging System (IVIS) monitored that strain Z1‐26 could survive and colonize in the gills and abdomen of crucian carp. The effects of dietary supplementation with strain Z1‐26 were evaluated with respect to the growth performance, antioxidant capacity, and immune response of crucian carp. The results showed that the Z1‐26‐fed fish had a significantly higher growth rate than the fish fed the control diet. The immune and antioxidant parameters revealed that the non‐specific immune indicators (AKP, SOD, and LZM) of the serum, the expression of immune‐related genes ( IgM, C3, and LZM ), and antioxidant‐related genes ( Nrf2 and Keap1 ) of the immune organs were significantly increased, whereas the expression of pro‐inflammatory factors ( IL‐1β, IL‐8, and TNF‐α ) of the immune organs was significantly down‐regulated in crucian carp fed strain Z1‐26 compared with fish fed a control diet. Moreover, fish fed with Z1‐26 supplemented diets showed a significantly improved survival rate after Aeromonas hydrophila infection. In addition, the whole genome analysis showed that strain Z1‐26 possesses 28 gene clusters, including 6 polyketide synthetase (PKS), 4 non‐ribosomal peptide‐synthetase (NRPS), 1 bacteriocin, and 1 lantipeptide. In summary, these results indicated that strain Z1‐26 could improve the growth performance and disease resistance in crucian carp, and has the potential to be developed as a candidate probiotics for the control of bacterial diseases in aquaculture.