Structural characterization and anti-inflammatory effects of an arabinan isolated from Rehmannia glutinosa Libosch. (1st March 2023)

• Record Type: Journal Article
• Authors: Zhang, Han; Yue, Yimin; Zhang, Qian; Liang, Lanyuan; Li, Chuanqiu; Chen, Ying; Li, Wei; Peng, Mingming; Yang, Mengru; Zhao, Mantong; Cao, Xia; Zhong, Luyang; Du, Jieyong; Wang, Yi; Zhou, Xi; Shu, Zunpeng
• Is Part Of: Carbohydrate polymers. Volume 303(2023)
• Publication Date: 2023-03-01
• Subjects: AFM atomic force microscopy -- Carboxy-H2DCFDA 5-(and-6)-carboxy-2′, 7′ -dichlorodihydrofluorescein diacetate -- CCRC the Complex Carbohydrate Research Center -- DAPI 4′-6-Diamidino-2-phenylindole -- DCFH-DA 2′, 7′-dichlorofluorescin diacetate -- DEAE-FF DEAE-Sepharose Fast Flow -- DMSO dimethyl sulfoxide -- Dpf days post fertilization -- FBS foetal bovine serum -- FT-IR Fourier transform infrared (spectroscopy) -- GC–MS gas chromatography-mass (spectrometry) -- Hpf hours post fertilization -- HPGPC high-performance gel permeation chromatography -- IL Interleukin -- LPS lipopolysaccharide -- MALDI-TOF-MS matrix-assisted laser desorption ionization time-of-flight mass -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- MW Molecular weight -- NMR Nuclear magnetic resonance -- PMAA partially methylated alditol acetate -- PMP 1-Phenyl-3-methyl-5-pyrazolone -- qRT-PCR Quantitative real-time PCR -- Rehmannia glutinosa Libosch R. glutinosa -- ROS Reactive oxygen species -- RPMI Roswell Park Memorial Institute -- TNF-α tumor necrosis factor-α -- UV–vis ultraviolet–visible; Rehmannia glutinosa Libosch. -- Arabinan -- Anti-inflammatory effect -- ROS-NF-κB pathway; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120441
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Considering that natural polysaccharides are potential anti-inflammatory agents, in this study, an arabinan (RGP70–2) was isolated and purified from Rehmannia glutinosa Libosch. ( R. glutinosa ) and its structure was characterized. RGP70–2 was a homogeneous polysaccharide with a molecular weight of 6.7 kDa, with the main backbone comprising →5)- α -L-Ara f -(1→, →3)- α -L-Ara f -(1→, →2, 3, 5)- α -L-Ara f -(1→, and →2, 5)- α -L-Ara f -(1 → linkages and the side chain comprising an α -L-Ara f -(1 → linkage. In vivo experiments showed that RGP70–2 inhibited ROS production and downregulated the expression of pro-inflammatory cytokines (TNF- α, IL-1 β, and IL-6). In vitro experiments showed that RGP70–2 decreased levels of pro-inflammatory cytokines, inhibited ROS production, and attenuated NF- κ B-p65 translocation from the cytoplasm to the nucleus. Our results showed that RGP70–2 may delay inflammation by regulating the ROS-NF- κ B pathway. Thus, RGP70–2 has potential applications as an anti-inflammatory agent in the biopharmaceutical industry. Graphical abstract: Unlabelled Image