Injectable remodeling hydrogels derived from alendronate-tethered alginate calcium complex for enhanced osteogenesis. (1st March 2023)

• Record Type: Journal Article
• Authors: Kim, Han-Sem; Jang, JunHwee; Oh, Jun-Sung; Lee, Eun-Jung; Han, Cheol-Min; Shin, Ueon Sang
• Is Part Of: Carbohydrate polymers. Volume 303(2023)
• Publication Date: 2023-03-01
• Subjects: NaAlg Sodium alginate -- Aln Sodium alendronate -- CaAlg Alginate hydrogel -- AlA Aln-tethered alginate -- AlACa Aln-tethered alginate calcium complex -- AlAA Mixture of AlACa and NaAlg hydrogel -- EDC-HCl 1-ethyl-3, 3-dimethyl aminopropyl carbodiimide hydrochloride -- NHS N-hydroxysuccinimide -- DS Degree of substitution -- hMSC Human mesenchymal stem cells -- ALP Alkaline phosphatase -- ARS Alizarin red S -- qRT-PCR quantitative real-time polymerase chain reaction -- OPN Osteopontin -- OCN Osteocalcin -- BSP Bone sialoprotein -- SR1/2 stress relaxation time; Alendronate -- Mesenchymal stem cell -- Injectable remodeling hydrogel -- Osteogenesis; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120473
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: A combination of hydrogel materials, and therapeutic agents have been actively reported to facilitate bone defect healing. However, conventionally hydrogels using cross-linker would result in low stability of the hydrogel itself, loss of agents during cross-linking, and complexity of use. In this study, alendronate was tethered to an AlA to improve its bone healing and drug-loading stability. AlA was further functionalized with Ca 2+ (AlACa). A mixture of AlACa and alginate formed AlAA hydrogel. The gelation time of AlAA was sufficient for injecting into the defect site. The hydrogel stiffness was controlled, while the stress-relaxation time was fixed. In vitro cell tests demonstrated that the AlAA promoted proliferation and differentiation behaviors. In particular, AlAA showed the best mechanical stiffness with appropriate stress-relaxation and cellular behavior, indicating that it would be beneficial as a scaffold in the bone tissue engineering field. Graphical abstract: Unlabelled Image