Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M. thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. Issue 1 (February 2023)

• Record Type: Journal Article
• Authors: Salih, Rabab; Banjanac, Katarina; Vukoičić, Ana; Gržetić, Jelena; Popović, Ana; Veljković, Milica; Bezbradica, Dejan; Marinković, Aleksandar
• Is Part Of: Journal of environmental chemical engineering. Volume 11:Issue 1(2023)
• Publication Date: 2023-02
• Subjects: Kraft lignin microspheres -- Immobilization support -- Laccase, decolorization of dye; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2022.109077
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50–90 µm with specific surface area of 39 m 2 /g, porosity εp 62, pore diameters of 4–20 nm and total pore volume of 0.14 cm 3 /g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5–7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles). Graphical Abstract: ga1 Highlights: Design of acrylic-modified lignin-based microspheres copolymerized with DEGDMA. Efficient decolorization of anthraquinone dyes with LMS-DEGDMA-laccase. The development of immobilized enzyme system for dye wastewater treatment. Perspective LMS-DEGDMA to be used as support for enzyme immobilization.