Exploring and comparing the impacts of low temperature to endogenous and exogenous partial denitrification: The nitrite supply, transcription mechanism, and microbial dynamics. (February 2023)

• Record Type: Journal Article
• Authors: Peng, Zhihao; Zhang, Qiong; Li, Xiyao; Wang, Shuying; Peng, Yongzhen
• Is Part Of: Bioresource technology. Volume 370(2023)
• Publication Date: 2023-02
• Subjects: Partial denitrification -- Low temperature -- Nitrite transformation rate -- Transcription mechanism -- Microbial dynamics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128568
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Low temperature favored higher NTR for two systems. In the long term, NTR in ExPD was 38.2% higher than EdPD. Higher NTR in low temperature originated from more inhibition of nirSK than narG. Less microbial dynamics and stronger cold-adaptation to EdPD, compared to ExPD. Low temperature caused great community dynamics and granule reduction to ExPD. Abstract: Nitrite supply was pretty significant to exogenous or endogenous partial denitrification (ExPD or EdPD) for their combination with anammox in removing nitrogen. This study investigated how temperature impacted the nitrite supply of ExPD and EdPD, through long-term experiments in two 10 L sequencing batch reactors (SBRs) and 12 batch temperature tests, with sodium acetate as organic. It was demonstrated that low temperature (5–15 °C) favored higher nitrite transformation rate (NTR) for two systems (1.1–1.3 and 1.1–1.2 times higher separately), and ExPD owned higher nitrite-supply ability than EdPD (32.8 % higher NTR). Moreover, quantitative reverse transcription PCR and 16srDNA sequencing were conducted, exploring the inherent mechanism and microbial dynamics. Results presented that more inhibition to transcription and translation of nirSK genes than narG in low temperature induced higher NTR. Besides, compared with ExPD, less microbial dynamics and granule size reduction occurred to EdPD, which was more capable of adapting to low temperature.